Investigating pH-Dependent Modulations in Haemoglobin Response to Linoleic Acid: A Spectroscopic Analysis

This study investigates the pH-dependent modulations in haemoglobin response to linoleic acid, employing a spectroscopic analysis across HbAA, HbAS, and HbSS variants. Concentration-dependent effects of linoleic acid at varying pH levels reveal nuanced behaviours in absorbance, peak maintenance, and spectra characteristics. At pH 7.2, HbAA and HbSS display increased absorbance with peak maintenance, while HbAS exhibits gradual absorbance rise. pH 5.0 induces absorbance increase in all variants, with maintained peaks at 415nm and gradual decrease at the oxy-band region. Variants exhibit distinct spectra characteristics, emphasizing the need for tailored approaches in clinical management. Findings align with empirical literature, emphasizing haemoglobin's multifunctionality. Implications span clinical considerations, dietary influences, and broader insights into haemoglobin stability. Suggestions for future studies propose molecular explorations, disease-specific investigations, computational modelling integration, longitudinal studies, and intervention strategy explorations. This study contributes to the evolving understanding of haemoglobin responses to environmental factors, laying groundwork for personalized approaches in healthcare and dietary recommendations.