Analysis of Chlorogenic Acid Oxidation Pathway in Simulated Enzymatic Honeysuckle Browning System by High Performance Liquid Chromatography and Mass Spectrometry

Purpose: To investigate the pathways involved in the oxidation of chlorogenic acid (CA) and phenol metabolism in honeysuckle buds.Methods: A model that mimics CA oxidation by honeysuckle polyphenol oxidase (PPO) by controlling the reaction temperature or reaction duration was employed, and the resulting products were analyzed by high-performance liquid chromatography followed by mass spectrometry (LC-MS).Results: The pathway of CA oxidation by PPO in honeysuckle involves CA, hydroxyl - CA, CA-oquinone derivatives, and CA-p-quinone derivatives.Conclusion: CA oxidation and the enzymatic browning reaction significantly impact the quality of honeysuckle and other fresh agricultural products.Keywords: Honeysuckle, Chlorogenic acid, Enzymatic browning, Mimic system, Oxidation pathway, High-performance liquid chromatography-mass spectrometry