Dysregulated Circulating micro RNAs Markers: New Evidence into Expression Pattern in Children with T1D among Egyptian Population

Abstract Background miRNAs are gaining access as promising markers for a variety of autoimmune disorders yet, deviations between individuals at risk or developed T1D remains to be thoroughly explored. We aimed to study the pattern of miRNA expression profiling in plasma obtained from patients with T1D and the matched control subjects. Methods Equally divided numbers of T1D patients (90) and healthy-matched control children (90) were analyzed for their expression profile of plasma miRNAs; miR-101-5p, 146-5p, 21-5p, miR-375, miR-126, and Let7a-5p by reverse transcriptase (RT-PCR) through quantitative real-time technique. Results The two studied groups were significantly different in respect to their biochemical parameters; FBG, 2hpp, and HbA1c levels (p< 0.05). Among the deregulated molecules, miR-101, miR-21 and-375 were highly expressed, whereas, miR-146-5p, miR-126, and miR-Let7a-5p showed significantly low levels of expression in patients compared to control subjects (p < 0.05). MiR-101, miR-146 was significantly correlated to the age at diagnosis of T1D and disease duration respectively. Furthermore, miR-126, -Let7a-5p showed a significant negative correlation with meanA1C values, the matter that was confined by multivariate analysis. Conclusion Dysregulation of the above mentioned micro RNAs pointed out to their pivotal role to be important biomarkers for T1D development.