DNA extraction for long-read sequencing of bacteria v1

Utilisation of long-read sequencing can reliably generate complete bacterial genomes. Here, we present a DNA extraction method which introduces minor modifications to the DNeasy® UltraClean® Microbial Kit (Qiagen) to generate DNA suitable for long-read sequencing. When sequenced, the extracted DNA should yield median read lengths greater than 7 kb. In addition, this protocol uses cultures growing on solid media as a starting point which will be useful for laboratories that do not routinely use liquid cultures.