Antioxidant and cytotoxic activity of ethyl acetate extract from Thermomyces lanuginosus and Aspergillus nidulans isolated from rhizospheric region of peanut (Arachis hypogaea)

Abstract Plant rhizospheric region is a good habitat of microbial communities that producing important secondary metabolites and essential bio products such as antioxidants and cytotoxins. Rhizospheric fungi are the best example for these communities. Thermomyces lanuginosus and Aspergillus nidulans were isolated from rhizospheric region of Arachis hypogaea and characterized morphologically and confirmed with Molecular genetic using (ITS) region. Ethyl acetate extract was a common solvent used in extraction and separation. Gas Chromatography Mass Spectrophotometer (GC-MS) was carried out to identify these fungal extracts and estimate their content . DPPH Scavenging was used for Screening of Antioxidant Activity and HeLa (Cervical Cancer), Colo-205 (Colorectal cancer) and MCF-7 (Breast Adenocarcinoma) for determaining Cytotoxicity and cell Viability.