Non-Enzymatic Generation of Placenta Single Cells from Third Trimester Human Placenta v1

The placenta is a heterogeneous and complex organ with multiple cell types, posing a challenge for the field of maternal-fetal medicine to implement single-cell technologies for a deeper characterization of this essential organ. Several protocols use enzymes to digest the tissue and generate single cell suspension, but this approach has several shortcomings including the loss and reduced viability of cells. In this study, we describe a non-enzymatic approach to generate single cell suspensionfrom placental tissue with high yield and viability for single cell RNAsequencing.