Anti-urolithiatic effect of a polyherbal formulation against sodium oxalate-induced urolithiasis in rats

Abstract The present study assesses the role of polyherbal formula (LACTN) against sodium oxalate (NaOx) - stimulated urolithiasis prophylactic and curatively. Forty eight rats were separated into the following regimen’s groups. Groups I, II, III, and IV (prophylactic groups) divided as follows; Group I (control group) received saline as vehicle. Group II (urolithiatic group) received 70 mg NaOx / kg b.wt, i.p. Groups III and IV received LACTN and Cystone prophylactic (500 and 750 mg/kg b.wt, respectively) from 1st day to 7th day of urolithiasis induction. Groups V, VI, VII, and VIII (curative groups); these divided as the prophylactic regimes but LACTN and Cystone administered from 7th day to 14th day of urolithiasis induction. Urolithiatic rats co/post treated with LACTN and Cystone elevated the urinary volume significantly (P<0.05). Also, significant decreased (P<0.05) were observed in the serum and urine calcium and phosphate concentrations beside the increased magnesium value in urolithiatic rats co/post treated with LACTN. LACTN significantly (P<0.05) ameliorate serum and urine creatinine, urea, and uric acid concentration. Moreover, urolithiatic rats co/post treated with LACTN significantly (P<0.05) diminished MDA and increase GSH, GST, SOD, and CAT compared with urolithiatic rats. In conclusion, LACTN has anti-lithogenesis effect that may be due to its ability to remove the already presented stone and/or preventing the generation of extra calcium oxalate crystals. This action may be due to its components’ synergetic action that may provide encouraging combined formula to prevent/treat urolithiasis.