A strong Association between Plasminogen Activator Inhibitor Type - 1 (-657 4G/5G PAI-1) and the Development of Pre-eclampsia

Abstract Introduction: Pre-eclampsia (P-EC) is a multisystem disorder of pregnancy, characterized by new-onset hypertension and proteinuria. Deregulation of the coagulation cascade and hypofibrinolysis appears to play a role in the development of P-EC. Methods: About 140 Egyptian pregnant women with a diagnosis of P-EC (patients' group) and 100 normotensive pregnant women not complicated by P-EC (a healthy control group) were collected. The genomic DNA was extracted from peripheral blood. Single-nucleotide polymorphisms were genotyped using ARMS-PCR. The serum was used for biochemical parameters measurement. Results: There is a strong significant (P < 0.0001) difference between PAI-1 5G/4G polymorphism among the patients and the control groups in the codominant model, high significant (P = 0.004) in the dominant model, high significant (P = 0.009) in the recessive model. Additionally, superoxide dismutase activity and reduced glutathione levels were decreased in pre-eclamptic women when compared to normotensive ones. On the other hand, malondialdehyde and glutathione peroxidase levels were increased in the pre-eclamptic group when compared with normalized pregnant ones. Conclusion: Our results showed that the mutated PAI-15G/4G polymorphisms may be associated with the development of P-EC in Egyptian women.