Enterovirus (EV) D68 real-time RT-PCR (EV-D68-TM2018) v2

This protocol aims to amplify enterovirus (EV) D68 viruses but not other viruses. This protocol is modified from a previously published method cited below. Details of the modification are included in the method. The oligonucleotides target the 5'UTR noncoding region. This is a qualitative test for investigating EV-D68 infection of humans. The test has identified both the historical EV-D68 strains and contemporary strains which has been confirmed by subgenomic sequencing of partial 5'UTR-VP2 and partial VP1 regions. Culture of the virus is not required as this assay is capable of detecting EV-D68 direct from extracted clinical samples.