Whole genome amplification of dengue virus type 1 - 4 v1

Dengue virus is one of the most often imported tropical viral infection in Hungary. For next-generation whole genome sequencing of dengue virus serotypes 1 - 4, a one-step reverse transcription PCR assay was developed. PCR amplicons can be used for further amplicon based sequencing protocols on Illumina MiSeq platform. The genome of the dengue virus is a single-stranded, positive-sense, capped RNA of approximately 10–11 kb in length. The whole genome amplification was carried out by five overlapping PCR amplicons, each one is approximately 2500 nucleotide in length. For ampification of the four serotypes, four different primer sets were designed by Geneious Prime (version 2021.2.2) primer design tool.