Micellar Enhanced Spectrofluorimetric Determination of Diphenylpyraline HCl and Dual Detection Cyclodextrin Micellar Liquid Chromatographic Method for Simultaneous Determination of Diphenylpyraline HCl, Paracetamol and Caffeine in Dosage Form

Abstract Highly sensitive micellar spectrofluorimetric method (Method I) has been developed and validated for the determination of diphenylpyraline HCl in pharmaceutical tablets and in plasma. Sodium dodecyl sulfate at pH 5 enhances the fluorescence intensity of diphenylpyraline at 286 nm that allow its determination at nano-level in plasma with mean percent recovery ± S.D of 99.719 ± 0.338. In addition, Green cyclodextrin micellar liquid chromatographic method (Method II) has been developed and validated for simultaneous determination of diphenylpyraline, paracetamol and caffeine using cyclodextrin micellar mobile phase consisted of 30 mM Brij*35, 0.5 mM hydroxypropyl β-cyclodextrin and phosphate buffer pH 4: MeOH (95:5, %v/v) that allows their simultaneous determination with enhanced spectrofluorimetric detection of diphenylpyraline. Method II was successfully applied for the simultaneous determination of diphenylpyraline, paracetamol and caffeine in a ternary laboratory prepared mixture containing all possible excipients with mean percent recoveries ± S.D of 100.176 ± 1.008, 101.166 ± 0.415 and 100.708 ± 1.836, respectively. The methods are verified to have excellent greenness.