Immunological profile of dengue markers in patients during the 2017 epidemic period in Bobo Dioulasso.

Abstract Introduction: The purpose of our study is to evaluate the immunological profile of dengue markers in patients suspected during the 2017 epidemic period in Bobo-Dioulasso. Methods and patients: This is a cross-sectional retrospective study of 367 subjects over a period of 03 months. Samples and clinical information come from suspected cases in the Hauts-Bassins health area. Whole blood had been taken from two tubes. A dry tube for immunochromatographic detection of antigen NS1, Immunoglobulin M and G. Another tube with EDTA for blood count by flow cytometry. The SD BIOLINE Dengue Duo® Kit was used for the diagnosis of dengue fever. Results: In general, we obtained a seroprevalence of 35.42%. The proportion of infected males and females was 19.61% and 15.80%, respectively. The distribution of direct and indirect markers in patients was 20.43% for AgNS1; 12.53% for IgG and 1.08% for IgM. Of the subjects in the study 39.39% had thrombocytopenia and 12.12% had leukopenia. Conclusion: In view of the results obtained, dengue fever is a real health problem in Burkina Faso. It also shows that direct markers of early diagnosis of dengue fever are more common than indirect markers. Therefore, ultrasensitive direct diagnostic tests should be used more extensively in the dengue diagnostic algorithm.