{# Audit 04/10/2026 : « autre » n'est pas un code de langue ; SPHAERO n'est pas l'éditeur des documents qu'elle héberge ou référence. #} {# citation_pdf_url doit mener à un PDF : un lien vers une page DOI est pénalisé par Google Scholar (avant : tout lien externe). #}
Accès ouvert

Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Article scientifique 2017 Anglais

Résumé

Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S- and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and characterise a novel S. aureus leukocidin; LukPQ. LukPQ is encoded on a 45 kb prophage (ΦSaeq1) found in six different clonal lineages, almost exclusively in strains cultured from equids. We show that LukPQ is a potent and specific killer of equine neutrophils and identify equine-CXCRA and CXCR2 as its target receptors. Although the S-component (LukP) is highly similar to the S-component of LukED, the species specificity of LukPQ and LukED differs. By forming non-canonical toxin pairs, we identify that the F-component contributes to the observed host tropism of LukPQ, thereby challenging the current paradigm that leukocidin specificity is driven solely by the S-component.

Citer ce document

Koop, G., Vrieling, M., Storisteanu, D., Lok, L., Monie, T., Wigcheren, G., Raisen, C., Ba, X., Gleadall, N., Hadjirin, N., Timmerman, A., Wagenaar, J., Klunder, H., Fitzgerald, J., Zadoks, R., Paterson, G., Torres, C., Waller, A., Loeffler, A., Loncaric, I., Hoet, A., Bergström, K., Martino, L., Pomba, C., Lencastre, H., Slama, K., Gharsa, H., Richardson, E., Chilvers, E., Haas, C., Kessel, K., Strijp, J., Harrison, E., Holmes, M. (2017). Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus. https://doi.org/10.1038/srep40660

Accès au document

Texte intégral en lecture en ligne, réservé aux abonnés SPHAERO et aux membres de l'institution. Se connecter

Voir l'article sur le site de la revue

Auteur(s)

Gerrit Koop Manouk Vrieling Daniel M. L. Storisteanu Laurence S. C. Lok Tom P. Monie Glenn F. van Wigcheren Claire Raisen Xiaoliang Ba Nicholas Gleadall Nazreen F. Hadjirin Arjen J. Timmerman Jaap A. Wagenaar H. Klunder J. Ross Fitzgerald Ruth N. Zadoks Gavin K. Paterson Cármen Torres Andrew S. Waller Anette Loeffler Igor Loncaric Armando E. Hoet Karin Bergström Luisa De Martino Constança Pomba Hermı́nia de Lencastre Karim Ben Slama Haythem Gharsa Emily J. Richardson Edwin R. Chilvers Carla de Haas Kok van Kessel Jos A. G. van Strijp Ewan M. Harrison Mark A. Holmes

Statistiques

Consultations : 4

Téléchargements : 0