One-Step Genotyping Protocol for Detection of Plasmodium falciparum hrp2 Gene Deletions v1

The emergence of Plasmodium falciparum parasites with deletions in the hrp2 gene poses a significant challenge to malaria diagnosis, particularly for rapid diagnostic tests (RDTs) that rely on HRP2 antigen detection. Reliable and scalable methods are therefore essential for monitoring the prevalence and distribution of these deletions. This protocol describes a rapid and simplified one-step PCR-based genotyping approach for the detection of hrp2 gene deletions in P. falciparum. The method is designed to streamline laboratory workflows by reducing processing time while maintaining high sensitivity and specificity. It incorporates appropriate positive, negative, and blank controls to ensure assay validity and reproducibility. Intended for use by trained personnel with a background in molecular biology, this protocol supports malaria research and surveillance activities. It is particularly suitable for implementation in endemic settings where timely and accurate detection of hrp2-deleted parasites is critical for guiding diagnostic strategies and public health interventions.