Plant Growth-Promoting Rhizobacteria Alleviate High Salinity Impact on the Halophyte Suaeda fruticosa by Modulating Antioxidant Defense and Soil Biological Activity

Plant growth-promoting rhizobacteria (PGPR) are considered as bio-ameliorators that confer better salt resistance to host plants while improving soil biological activity. Despite their importance, data about the likely synergisms between PGPR and halophytes in their native environments are scarce. The objective of this study was to assess the effect of PGPR (Glutamicibactersp. andPseudomonassp.) inoculation on biomass, nutrient uptake, and antioxidant enzymes ofSuaeda fruticosa, an obligate halophyte native in salt marshes and arid areas in Tunisia. Besides, the activity of rhizospheric soil enzyme activities upon plant inoculation was determined. Plants were grown in pots filled with soil and irrigated with 600 mM NaCl for 1 month. Inoculation (either withPseudomonassp. orGlutamicibactersp.) resulted in significantly higher shoot dry weight and less accumulation of Na+and Cl–in shoots of salt-treated plants.Glutamicibactersp. inoculation significantly reduced malondialdehyde (MDA) concentration, while increasing the activity of antioxidant enzymes (superoxide dismutase; catalase; ascorbate peroxidase; and glutathione reductase) by up to 100%. This provides strong arguments in favor of a boosting effect of this strain onS. fruticosachallenged with high salinity.Pseudomonassp. inoculation increased shoot K+and Ca2+content and lowered shoot MDA concentration. Regarding the soil biological activity,Pseudomonassp. significantly enhanced the activities of three rhizospheric soil enzymes (urease, ß-glucosidase, and dehydrogenase) as compared to their respective non-inoculated saline treatment. Hence,Pseudomonassp. could have a great potential to be used as bio-inoculants in order to improve plant growth and soil nutrient uptake under salt stress. Indole-3-acetic acid concentration in the soil increased in both bacterial treatments under saline conditions, especially withGlutamicibactersp. (up to +214%). As a whole,Glutamicibactersp. andPseudomonassp. strains are promising candidates as part of biological solutions aiming at the phytoremediation and reclamation of saline-degraded areas.