Evaluation of Biochemical, Hematological and Oxidative Parameters in Mice exposed to the Binapacryl

Abstract We evaluated the toxicity of hepatic, hematological, and oxidative effects of binapacryl on female albino mice. The animals were treated orally with 50, 100, 150 and 200 mg/kg body weight of the binapacryl, on a daily basis for a period of 15 days. Distilled water was used as control treatment. Samples of blood and hepatic tissue were collected at the end of the treatment. Hepatotoxicity was monitored by quantitative analysis of the serum enzymes ALT, AST, γ-GT, urea and creatinine. We also investigated liver tissues histopathologically. Alterations of hematological parameters were monitored by RBC, WBC, hemoglobin, hematocrit, MCV, MCH, and MCHC. Significant increases in the levels of hepatic enzymes (ALT, AST, and γ-GT) were observed for both binapacryl treatments, but no considerable differences were found by histological analysis. The hematological parameters showed significant alterations (200mg/kg body weight) with reductions of RBC, hematocrit, and hemoglobin, together with a significant increase of MCV. There was an important increase in lipid peroxidation at both dosage levels, together with an NPSH decrease in the hepatic tissue, and significant changes in these parameters were observed only at the higher dose rate. The results of this study indicate that binapacryl can promote hematological and hepatic alterations, even at subacute exposure, which could be related to the induction of reactive oxygen species.