Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks

AIM: Enteritidis (SE) specific antibodies in chicken sera. MATERIALS AND METHODS: protein A was used as capture antibody at the test line (T) of a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit antibodies were used as capture antibody at the control line (C) of the NC strip in the lateral flow layout device. RESULTS: antigen were 88.8%, 90%, and 89%, respectively. CONCLUSION: The developed test is a simple field rapid test of high sensitivity, specificity, and accuracy that can improve and facilitates rapid field surveillance of salmonellosis among chickens.